Summary
Type: SPE
Application #: 300520
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, then 2 column volumes 0.05 N formic acid pH 3
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
2 x 500 μl 0.05 N formic acid / acetonitrile (3 : 1, v/v); dry column 5 min
under vacuum
Elution:
2 x 500 μl methanol
Authors: Macherey-Nagel
Source: LC department